Characterization of polymorphisms in CFI and ARMS genes and their association with exudative age-related macular degeneration in Algerian patients

Increasing evidence shows that polymorphisms in CFI and ARMS2 genes can influence exudative age-related macular degeneration (nAMD) risk. The aim of this study was to assess the role of CFI rs10033900 and ARMS2 rs3750846 polymorphisms in susceptibility to nAMD for the first time in the Algerian population. A total of one hundred twenty four controls and seventy two nAMD cases were included in the present study. Genomic DNA was extracted from venous blood leukocytes. CFI rs10033900 and ARMS2 rs3750846 variants were determined by using the real‑time polymerase chain reaction method. Differences in allele and genotype distribution between the cases and controls were tested with adjustment for age by logistic regression analysis. A stratification of case and control groups by age (<65 or ≥65) and by gender (male and female) was also performed. Statistical analyses were done using SPSS21.0. No statistically significant association was observed between CFI rs10033900 and ARMS2 rs3750846 polymorphisms and nAMD risk (p>0.05 for all comparisons). Stratification by age and gender did not show any significant association between these two polymorphisms and nAMD in a sample of the Algerian population. In our study, CFI rs10033900 and ARMS2 rs3750846 polymorphisms did not predispose alone to nAMD in our population. This study is a contribution to the enrichment of the bank data concerning the CFI and ARMS2 genes, reporting, for the first time, the allelic and genotypic frequencies of these genes polymorphisms characterizing the Algerian population.


INTRODUCTION
Age-related macular degeneration (AMD) is the principal cause of central visual impairment in the elderly population [1]. In advanced stage, AMD could evolve into exudative

MATERIALS AND METHODS
In total, One hundred ninety six subjects were enrolled in this case-control study: One hundred twenty four healthy controls and seventy two patients with exudative AMD. Patients were recruited from Hassani Abdelkader Hospital (Sidi Bel Abes, Algeria) whereas control subjects were volunteer's healthy people. All patients and control subjects enrolled in our study provided informed consent, and the work was approved by the ethics committee at the Algerian Thematic Agency for Research in Health Sciences (The Code of Ethics of the ATRSSV for experiments in humans STCE 164), available on https://atrss.dz/std.php?id=749 . Diagnosis of nAMD was confirmed by an ophthalmologic examination and was made based on bestcorrected visual acuity (BCVA) test and Snellen chart, slit-lamp examination, dilated fundus examination, fluorescein angiography (FA), and measurement of central retinal thickness (CRT) using optical coherence tomography (OCT). People over the age of 50 who had been diagnosed with nAMD after undergoing full ocular examinations and had choroidal neovascularization (CNV) in one or both eyes were included in this study. CNV resulting from the other causes and the other retinal disorders were excluded. Subjects over the age of 50 with no clinical evidence of nAMD and free of retinal disorder, or any other ocular pathology were recruited as control samples.
The genomic DNA was extracted from venous blood sample of each patient; using the Salting Out method. CFI rs10033900 and ARMS2 rs3750846 SNPs genotyping was performed using the real-time polymerase chain reaction method (RT-PCR); SNP Genotyping Assay (CFI rs10033900 Assay ID: C__34681305_20/ARMS2 rs3750846 Assay ID: C_27473788_10 ) were used in an appropriate RT-PCR mixture containing 2X TaqMan Universal Master Mix (Thermo Fisher Scientific, France), and nuclease-free water. PCR conditions were as following: 10 min at 95°C; 40 cycles (15 s at 95°C, and 1 min at 60°C).
Statistical analysis was performed using the SPSS 21.0 software. CFI rs10033900 and ARMS2 rs3750846 polymorphisms were tested for Hardy-Weinberg analysis. We performed Student's t-test for numerical variables and Chi-squared test for categorical variables to compare characteristics between nAMD and control groups. The distribution of genotypes and alleles in the nAMD patients and control groups was compared using logistic regression analysis measured by odds ratio (OR) and 95% confidence interval (CI) to estimate the impact of the two studied polymorphisms on the exudative AMD development. Statistical analysis was adjusted by age. Difference was considered significant when P value was less than 0.05. Sample size was determined using Quanto software version 1.2, for an unmatched case-control study with 95% confidence interval and 70 to 80% power.

RESULTS
Cases and controls characteristics are given in Table 1. A significant difference were observed in age between cases (72.0±9.0 years) and controls (62.8±8.9 years). However, gender did not reveal any significant difference between the two groups (p=0.46) (Table1). Genotypes and alleles frequencies of CFI rs10033900 and ARMS2 rs3750846 polymorphisms were conformed to Hardy-Weinberg equilibrium in our population (p>0.05). Frequencies of CFI rs10033900 and ARMS2 rs3750846 genotype and allele distribution are presented in Table 2. The C risk allele of the CFI rs10033900 polymorphism had a frequency of 0.61 in the control group, whereas, the C allele frequency of ARMS2 rs3750846 polymorphism was 0.24 in the same group. Difference in genotype (CC, CT, and TT) and allele (C/T) frequencies between cases and controls were not statistically significant for either CFI rs10033900 polymorphism (alleles: p=0.9, genotypes: p=0. 8  For analysing the results of table 2 regarding the age, alleles and genotypes distribution of rs10033900 and rs3750846 polymorphisms were compared between subjects under 65 years age and over 65 years age. Results indicated no significant variation in the alleles and genotypes diversity of these two polymorphisms (Table 3).

DISCUSSION
In this study, the C risk allele of CFI rs10033900 and ARMS2 rs3750846 SNPs was found in 61% and 24% of our sample respectively. This frequency was comparable with that reported in Europeans such as UK, England, Scotland populations, and in cohort in China, and Southeastern United States [7,[16][17][18]. However, the C allele of CFI rs10033900 SNP was twice as high in our population as it was in the Chinese (0.32) and Japanese population (0.39) [19,20]. In addition, Shin et al. (2021) reported that this allele was detected in 66% of Africans, 53% of Americans, 54% of European, but only in 31% of South Asians, whereas that of ARMS2 rs3750846 SNP was detected in 26% of Africans, 25% of Americans, 19% of Europeans, suggesting the existence of genetic differences among racial and ethnic groups, affecting consequently AMD prevalence, that was higher in East Asians than in Europeans due to genetic predisposition in East Asians [21].
CFI plays an important role as a regulatory protein in the alternative pathway of complement (APC) by converting C3b and C4b to an inactive form to reduce the C3 and C5 convertases formation, limiting otherwise the activation of APC and preventing tissue damage [4]. Further, Studies, including Wang et al. study, demonstrated a significant association of chromosomal 10q26 genes, especially ARMS2, with AMD, suggesting this gene as the most likely major AMD susceptibility gene [22]. ARMS2 protein was reported to initiates complement activation by binding to human apoptotic and necrotic cells membrane, as consequence, variants affecting this protein may have an impact on dysregulation of the complement system which is a significant driver of AMD pathogenesis [13]. Also, recently, Battu et al (2021) reported that ARMS2 protein levels were significantly higher in the AMD patients than in the control group, practically dry AMD patients, indicating that this protein may be involved in the pathogenesis of AMD [23]. Our results showed that there is no significant association of both CFI rs10033900 and ARMS2 rs3750846 gene polymorphisms with exudative AMD in our study, which is the first to investigate this relationship in an Algerian population. This was consistent with results of Cipriani et al. for the rs10033900 SNP in British (p=0.5) and Scottish group (p=0.7) and of that found in Chinese (p=0.8) and Turkish population (p=0.8) [10,16,24]. In contrast, Qian et al. identified this variant to be a risk-increasing factor associated to AMD in Chinese population [19]. Moreover, rs10033900 variant was reported to be a decreased risk factor of nAMD in Chinese (p=0.002) and Japanese population (0.003) [20,25]. Also, a large GWAS study indicated that ARMS2 rs3750846 SNP increase the risk of AMD in Europeans [3]. Recently, it was also shown in a large European consortium as a highest risk of advanced AMD [15]. Furthermore, in the study of Schick et al. (2020), the minor allele "C" was reported as an increased risk of late AMD (p≤0.0006) [14]. The present study had some limitations such as the small sample size and the possibility of other polymorphism risk factors was not included in the study. Therefore, an expanded study is required by incorporating a large sample, and investigating variants in other genes in order to give more evidence of a genetic contribution in the etiology of nAMD.
Finally, this study is a contribution to the enrichment of the bank data concerning the CFI and ARMS2 gene, reporting, for the first time, the allelic and genotypic frequencies of these genes polymorphisms characterizing the Algerian population.